Direct Detection of Bordetella pertussis and Bordetella parapertussis: Comparison of Polymerase Chain Reaction and Culture
نویسندگان
چکیده
منابع مشابه
Comparison of multiplex polymerase chain reaction, culture, and serology for the diagnosis of Bordetella pertussis infection.
BACKGROUND Accurate diagnosis of Bordetella pertussis infection is difficult. Polymerase chain reaction (PCR) tests are more sensitive than culture, but the reported sensitivity is variable. We prospectively compared the performance of culture, serology, and a multiplex PCR for the detection of B. pertussis. METHODS A total of 193 paired nasopharyngeal (NP) swab specimens were examined by bot...
متن کاملComparison of polymerase chain reaction, culture, and western immunoblot serology for diagnosis of Bordetella pertussis infection.
Polymerase chain reaction (PCR) amplification of the pertussis toxin promoter region was used to detect Bordetella pertussis infection in nasopharyngeal aspirates collected from 24 infants and children infected with pertussis and 13 adult contacts during an epidemiological study. The sensitivity of this PCR assay was approximately one bacterium, and the assay was specific for B. pertussis in te...
متن کاملmultiplex real-time pcr assay for detection and differentiation of bordetella pertussis and bordetella parapertussis.
background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...
متن کاملMultiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis
A triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis was developed. Three targets were used for amplification in a single tube: the insertion sequence IS481 and the pertussis toxin promoter region (ptxP) for B. pertussis, and the insertion sequence IS1001 for B. parapertussis. The performance of this PCR assay was evaluated in para...
متن کاملReal-time LightCycler PCR for detection and discrimination of Bordetella pertussis and Bordetella parapertussis.
Real-time PCR assays based on the LightCycler technology were developed for individual (simplex PCR) and simultaneous (duplex PCR) detection and discrimination of Bordetella pertussis and Bordetella parapertussis in clinical samples. The assays were evaluated with 113 specimens from patients with and without symptoms of pertussis. Results were compared to those from conventional culture and Taq...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of International Medical Research
سال: 2006
ISSN: 0300-0605,1473-2300
DOI: 10.1177/147323000603400405